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il 5  (R&D Systems)


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    Structured Review

    R&D Systems il 5
    Effect of IL-29 on eosinophil inflammatory cytokine secretion. (A) ELISA analysis <t>of</t> <t>IL-5</t> secretion in eosinophil supernatants; (B) ELISA analysis of IL-13 secretion in eosinophil supernatants. Eosinophils were stimulated with different concentrations of IL-29 (10, 25, 50, 100 ng/mL) for 24 hours. Data were obtained from 3 independent experiments and are presented as mean ± SD. * indicates p < 0.05.
    Il 5, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+5/Recombinant+Human+IL-5+Protein/pmc13113271-60-24-25
    Average 92 stars, based on 59 article reviews
    il 5 - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "Dose-dependent IL-29 activation of TLR4 signalling drives eosinophil infiltration in chronic rhinosinusitis with nasal polyps"

    Article Title: Dose-dependent IL-29 activation of TLR4 signalling drives eosinophil infiltration in chronic rhinosinusitis with nasal polyps

    Journal: Acta Otorhinolaryngologica Italica

    doi: 10.14639/0392-100X-A1222

    Effect of IL-29 on eosinophil inflammatory cytokine secretion. (A) ELISA analysis of IL-5 secretion in eosinophil supernatants; (B) ELISA analysis of IL-13 secretion in eosinophil supernatants. Eosinophils were stimulated with different concentrations of IL-29 (10, 25, 50, 100 ng/mL) for 24 hours. Data were obtained from 3 independent experiments and are presented as mean ± SD. * indicates p < 0.05.
    Figure Legend Snippet: Effect of IL-29 on eosinophil inflammatory cytokine secretion. (A) ELISA analysis of IL-5 secretion in eosinophil supernatants; (B) ELISA analysis of IL-13 secretion in eosinophil supernatants. Eosinophils were stimulated with different concentrations of IL-29 (10, 25, 50, 100 ng/mL) for 24 hours. Data were obtained from 3 independent experiments and are presented as mean ± SD. * indicates p < 0.05.

    Techniques Used: Enzyme-linked Immunosorbent Assay

    The interventional effect of TAK-242 Blocking TLR4 on IL-29 action. (A) Transwell assay showing changes in eosinophil migration after TLR4 blockade with TAK-242; (B) ELISA of IL-5 secretion after TAK-242 intervention; (C) ELISA of IL-13 secretion after TAK-242 intervention; (D) Western blot analysis of TLR4, p-NF-κB, and p-MAPK protein expression after TAK-242 treatment. Data are presented as mean ± SD. * indicates p < 0.05.
    Figure Legend Snippet: The interventional effect of TAK-242 Blocking TLR4 on IL-29 action. (A) Transwell assay showing changes in eosinophil migration after TLR4 blockade with TAK-242; (B) ELISA of IL-5 secretion after TAK-242 intervention; (C) ELISA of IL-13 secretion after TAK-242 intervention; (D) Western blot analysis of TLR4, p-NF-κB, and p-MAPK protein expression after TAK-242 treatment. Data are presented as mean ± SD. * indicates p < 0.05.

    Techniques Used: Blocking Assay, Transwell Assay, Migration, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing

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    Article Snippet: .. In vitro plasmablast differentiation Plasmablasts were generated in vitro by stimulation of CD43– B cells with 0.2 μM CpG (ODN 1826; InvivoGen) plus 10 ng/ml IL- 4 (404-ML-025/CF; R&D Systems) and 10 ng/ml IL-5 (405-ML025; R&D Systems). ..

    Article Title: In vivo CRISPR/Cas9 screens identify new regulators of B cell activation and plasma cell differentiation
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    Article Title: In vivo CRISPR/Cas9 screens identify new regulators of B cell activation and plasma cell differentiation.
    Article Snippet: .. In vitro plasmablast differentiation Plasmablasts were generated in vitro by stimulation of CD43– B cells with 0.2 μM CpG (ODN 1826; InvivoGen) plus 10 ng/ml IL- 4 (404-ML-025/CF; R&D Systems) and 10 ng/ml IL-5 (405-ML025; R&D Systems). ..

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    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

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    Article Snippet: For mouse cytokines, culture supernatant from eosinophil stimulation was used to detect granule proteins expressions, including eosinophil cationic protein (ECP, Abbkine, Cat# KTE71563 ) and eosinophil peroxidase (EPX, FineTest, Cat# EM6789). .. For mouse BALF cytokines expression, type2 inflammatory cytokines, including IL-4 (Mouse IL-4 DuoSet ELISA, R&D Systems, Cat# DY404-05), IL-5 (Mouse IL-5 DuoSet ELISA, R&D Systems, Cat# DY405-05) and IL-13 (Mouse IL-13 DuoSet ELISA, R&D Systems, Cat# DY413-05), were measured. ..

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    Expressing:

    Article Title: Airway immune profiles and therapeutic implications of IGF1 in eosinophilic granulomatosis with polyangiitis
    Article Snippet: For mouse cytokines, culture supernatant from eosinophil stimulation was used to detect granule proteins expressions, including eosinophil cationic protein (ECP, Abbkine, Cat# KTE71563 ) and eosinophil peroxidase (EPX, FineTest, Cat# EM6789). .. For mouse BALF cytokines expression, type2 inflammatory cytokines, including IL-4 (Mouse IL-4 DuoSet ELISA, R&D Systems, Cat# DY404-05), IL-5 (Mouse IL-5 DuoSet ELISA, R&D Systems, Cat# DY405-05) and IL-13 (Mouse IL-13 DuoSet ELISA, R&D Systems, Cat# DY413-05), were measured. ..

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    Image Search Results


    Effect of IL-29 on eosinophil inflammatory cytokine secretion. (A) ELISA analysis of IL-5 secretion in eosinophil supernatants; (B) ELISA analysis of IL-13 secretion in eosinophil supernatants. Eosinophils were stimulated with different concentrations of IL-29 (10, 25, 50, 100 ng/mL) for 24 hours. Data were obtained from 3 independent experiments and are presented as mean ± SD. * indicates p < 0.05.

    Journal: Acta Otorhinolaryngologica Italica

    Article Title: Dose-dependent IL-29 activation of TLR4 signalling drives eosinophil infiltration in chronic rhinosinusitis with nasal polyps

    doi: 10.14639/0392-100X-A1222

    Figure Lengend Snippet: Effect of IL-29 on eosinophil inflammatory cytokine secretion. (A) ELISA analysis of IL-5 secretion in eosinophil supernatants; (B) ELISA analysis of IL-13 secretion in eosinophil supernatants. Eosinophils were stimulated with different concentrations of IL-29 (10, 25, 50, 100 ng/mL) for 24 hours. Data were obtained from 3 independent experiments and are presented as mean ± SD. * indicates p < 0.05.

    Article Snippet: A total of 1×10 5 eosinophils were seeded in the upper chamber, while the lower chamber was filled with RPMI-1640 medium containing 10 ng/mL IL-5 (R&D Systems, 205-IL-010) as a chemoattractant.

    Techniques: Enzyme-linked Immunosorbent Assay

    The interventional effect of TAK-242 Blocking TLR4 on IL-29 action. (A) Transwell assay showing changes in eosinophil migration after TLR4 blockade with TAK-242; (B) ELISA of IL-5 secretion after TAK-242 intervention; (C) ELISA of IL-13 secretion after TAK-242 intervention; (D) Western blot analysis of TLR4, p-NF-κB, and p-MAPK protein expression after TAK-242 treatment. Data are presented as mean ± SD. * indicates p < 0.05.

    Journal: Acta Otorhinolaryngologica Italica

    Article Title: Dose-dependent IL-29 activation of TLR4 signalling drives eosinophil infiltration in chronic rhinosinusitis with nasal polyps

    doi: 10.14639/0392-100X-A1222

    Figure Lengend Snippet: The interventional effect of TAK-242 Blocking TLR4 on IL-29 action. (A) Transwell assay showing changes in eosinophil migration after TLR4 blockade with TAK-242; (B) ELISA of IL-5 secretion after TAK-242 intervention; (C) ELISA of IL-13 secretion after TAK-242 intervention; (D) Western blot analysis of TLR4, p-NF-κB, and p-MAPK protein expression after TAK-242 treatment. Data are presented as mean ± SD. * indicates p < 0.05.

    Article Snippet: A total of 1×10 5 eosinophils were seeded in the upper chamber, while the lower chamber was filled with RPMI-1640 medium containing 10 ng/mL IL-5 (R&D Systems, 205-IL-010) as a chemoattractant.

    Techniques: Blocking Assay, Transwell Assay, Migration, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing